- Doctor Octopus/Dr. Otto Octavius (Leader) (Defected)
- Kraven the Hunter/Sergei Kravinoff
- Crossbones/Lizard/Brock Rumlow (Unwillingly, Formerly)
- Vulture/Adrian Toomes (Unwillingly, Defected)
- Rhino/Alexander Sytsevich (Unwillingly, Defected)
- Each member's powers, physical appearance, or equipment is based on an animal listed below:
- Doctor Octopus' octopus tentacles.
- Kraven's lion vest.
- Scorpion's scorpion armor.
- Crossbones' lizard attributes (after being injected by Scorpion).
- Vulture's vulture armor/attributes.
- Rhino's rhinoceros attributes.
- This is the first Sinister Six group in any other comics and media to be an animal-themed version.
Background in other media
- In the comics, the Superior Six consisted of the Superior Spider-Man (Otto Octavius), Mysterio, Chameleon, Vulture, Electro, and Sandman.