Double Agent Venom is the sixth episode of the fourth season of Ultimate Spider-Man.
- Spider-Man/Peter Parker
- Agent Venom/Flash Thompson
- Scarlet Spider/Ben Reilly
- Iron Spider/Amadeus Cho (cameo)
- Power Man/Luke Cage (cameo)
- Cloak/ and Tandy Bowen (cameo)
- Dagger/Tyrone Johnson (cameo)
- Drake Bell as Spider-Man
- Diedrich Bader as Kraven the Hunter
- Matt Lanter as Agent Venom
- Scott Porter as Scarlet Spider
- Tom Kenny as Doctor Octopus
- Mark Hamill as Arnim Zola
- Max Mittelman as Rhino
The sonic machine used to remove the Venom Symbiote is similar in appearance to the machine that changed Norman Osborn into the Green Goblin in Spider-Man: Turn off the Dark.